Virus Culture Fundamentals: Methods and Strategies for Viral Propagation

Thursday, February 18, 2021 12:00 PM - 1:00 PM EDT


Viruses are pathogenic intracellular organisms that require living cells in order to multiply. The successful replication of these infectious particles in the laboratory depends on specific tools and processes; however, even the most experienced virologist can encounter issues when culturing different viral species. At ATCC, the Manufacturing Science and Technology (MSAT) Virology Department tackles new viral product manufacturing and troubleshooting and performs product improvement research. In this presentation, MSAT virologists will provide an in-depth look at the various methods employed in viral propagation and will go over the basic strategies used at ATCC to improve the quality of existing viral products.

Key Takeways:

  • ATCC cultures viruses with several basic propagation types (e.g., lytic, cell-associated, persistently infected cells, egg propagation).
  • Multiple tools and processes are used to optimize protocols, including multiplicity of infection (MOI) optimization.
  • ATCC authenticates and tests each virus to make sure we are distributing the highest quality products to our customers.
  • ATCC uses numerous strategies for troubleshooting (e.g., mycoplasma cleanup, failure to grow, adaptation to new host cells).

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